Identification of 0 - Alkyldihydroxyacetone Phosphate , O - Alkyldihydroxyacetone , and Diacyl Glyceryl

Biosynthesis of an O-alkyl analogue of phosphatidic acid and O-alkylglycerols via O-alkyl ketone intermediates by microsomal enzymes of Ehrlich ascites tumor.

Microsomal enzymes from Ehrlich ascites tumors synthesized 0-alkyl glyceryl ethers from hexadecanol and dihydroxyacetone phosphate in the presence of ATP, CoA, and Mg++. Glyceryl ether synthesis occurred via the formation of 0-alkyldihydroxyacetone phosphate and O-alkyldihydroxyacetone. Although NADPH inhibited the initial reaction of dihydroxyacetone phosphate with fatty alcohol, it was requir...

Reductase, phosphatase, and kinase activities in the metabolism of alkyldihydroxyacetone phosphate and alkyldihydroxyacetone.

Enzymic synthesis of O-alkyl bonds in glycerolipids.

We have found that 0-alkyl bonds in glycerolipids are synthesized by microsomal enzymes of preputial gland tumors when diiydroxyacetone-P or glyceraldehyde-3-P and 1 J4C long chain fatty alcohols are used as substrates; ATP, coenzyme A, and Mg++ are essential cofactors. The 0-alkyl moieties formed from the 1-“Cand 9,10-3H-labeled long chain fatty alcohols show that the fatty alcohol chains are ...

Biosynthesis of glycerolipids via acyldihydroxyacetone phosphate.

Acyldihydroxyacetone phosphate was first discovered as a lipid rapidly labelled by ’’Pi or by [p”P]ATP in guinea-pig liver mitochondria (Hajra & Agranoff, 1967,1968). This rapid labelling was due to the dephosphorylation and rephosphorylation of endogenous acyldihydroxyacetone phosphate in mitochondria (Hajra et al., 1968). Acyldihydroxyacetone phosphate was shown to be biosynthesized by the di...

The role of acyldihydroxyacetone phosphate, reduced nicotinamide adenine dinucleotide, and reduced nicotinamide adenine dinucleotide phosphate in the biosynthesis of O-alkyl glycerolipids by microsomal enzymes of Ehrlich ascites tumor.

Pahnitoyl dihydroxyacetone phosphate and [1-14C]hexadecanol were found to serve as precursors of 0-alkyl lipids in a microsomal system from Ehrlich ascites tumor. Both acyldihydroxyacetone phosphate and dihydroxyacetone phosphate were tested. The system required no CoA when acyldihydroxyacetone phosphate was used as the glycerol source, and significantly lower concentrations of acyldihydroxyace...